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. 2012 Jul 27;7(7):e40637. doi: 10.1371/journal.pone.0040637

Table 1. Data production.

CE1 CE2 JP (mean ± s.d.) CM (mean ± s.d.) HM1 HM2
Target region (Mb) 37.63 37.63 45.88 37.63 37.63 37.63
# of clean reads(Mb) 31.71 51.12 98.43±8.24 27.55±2.75 25.56 25.56
Reads mapped to humangenome(Mb) 18.12 28.05 55.68±6.07 23.54±2.30 23.58 23.35
(fraction of clean reads%) 57.13 54.86 56.48±2.06 85.46±1.06 92.26 91.37
Reads mapped to human genomeafter filter duplication(Mb) 17.66 27.30 48.44±4.93 22.22±2.23 22.74 22.42
(fraction of clean reads%) 55.69 53.40 49.19±2.56 80.67±1.68 88.97 87.72
Reads mapped to own genome(Mb) 27.02 45.92 88.22±8.09 24.22±2.15 23.58 23.35
(fraction of clean reads%) 85.20 89.83 89.58±1.19 88.01±1.79 92.26 91.37
Reads mapped to target region(Mb) 14.26 21.04 88.22±8.09 16.67±1.71 17.08 17.35
(fraction of clean reads%) 44.96 41.17 40.66±2.95 60.51±1.60 66.82 67.89
Mean depth of target region 30.72 45.77 70±8.52 35.03±3.40 35.41 36.14
Coverage of target region(%) 91.06 92.70 93.29±0.14 97.16±0.18 97.08 97.73
Capture specificity 52.77 45.83 45.36±2.84 68.78±2.21 72.43 74.30
Fraction of target covered > = 4X(%) 81.14 83.67 87.03±0.40 91.06±0.83 91.30 92.27
Fraction of target covered> = 10X(%) 67.59 72.32 78.80±0.86 78.41±2.38 79.09 79.91
Rate of nucleotide mismatch (%) 2.90 2.76 2.76±0.07 1.03±0.05 0.44 0.48

Summary of captured target sequence coverage for each non-human primate exome and two human exomes. The total size of the captured target is 37,627,322 bp for CE and 45,880,359 bp for JP. Each exome was compared to the human reference genome. Listed for each exome are the details of captured data including alignment, depth and coverage of target region. The exomes of all twenty one individuals were sequenced with a mean depth ≥28 fold on array designed target region (TR). Coverage of target region ranged from 91.06% to 97.73%, and 67.59% to 81.33% of sites in the target region were covered by more than 10 reads. Non-human primates were aligned to their own or closest genomes (rheMac2 was used as reference genome for crab-eating cynomolgus and Japanese macaque) to evaluate the capture efficiency.