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. 2012 Jul 27;7(7):e41486. doi: 10.1371/journal.pone.0041486

Table 1. Primers used for the construction of genetically modified full-length clones of FMDV.

Primer Sequencea(5′→3′) Nucleotide Position
HN-1F CAAGAAGTGATTGAGCGGGT 5274–5293
HN-1R CTTATCGTCATCGTCCTTATAGTC CTGGCGCCTCTTGCGCGCTT 5683–5702
HN-2F GACTATAAGGACGATGACGATAAG GACGCGGCTCTTGACGATGC 5613–5632
HN-2R GCGTTGTTGATCACGCCGAC 6324–6343
HN-3F CAGCCGGAACTGGCTCCGGAAGACCCGGAAGAC GTGCCCGGGAGGGAACAA 5736–5755
HN-3R GTCTTCCGGGTCTTCCGGAGCCAGTTCCGGCTG TTGCTCGGTGGGGGATCT 5683–5702
HN-4F AGGTGTCGAGTCACCCGATT 5308–5327
HN-4R GTTCCCTTCTTCATTCTCGC 6297–6316
a

Nucleotides encoding the FLAG and HSV tags are marked with italic type.