Table 1. Primers used for the construction of genetically modified full-length clones of FMDV.
Primer | Sequencea(5′→3′) | Nucleotide Position |
HN-1F | CAAGAAGTGATTGAGCGGGT | 5274–5293 |
HN-1R | CTTATCGTCATCGTCCTTATAGTC CTGGCGCCTCTTGCGCGCTT | 5683–5702 |
HN-2F | GACTATAAGGACGATGACGATAAG GACGCGGCTCTTGACGATGC | 5613–5632 |
HN-2R | GCGTTGTTGATCACGCCGAC | 6324–6343 |
HN-3F | CAGCCGGAACTGGCTCCGGAAGACCCGGAAGAC GTGCCCGGGAGGGAACAA | 5736–5755 |
HN-3R | GTCTTCCGGGTCTTCCGGAGCCAGTTCCGGCTG TTGCTCGGTGGGGGATCT | 5683–5702 |
HN-4F | AGGTGTCGAGTCACCCGATT | 5308–5327 |
HN-4R | GTTCCCTTCTTCATTCTCGC | 6297–6316 |
Nucleotides encoding the FLAG and HSV tags are marked with italic type.