Table 3. The OD450 value of anti-tag and anti-3ABC antibodies in mice infected with the 3A-tagged viruses and the parental virus.
| Virus | Animal No. | 3ABC | HSV | FLAG |
| FMDV-HSV | M1 | 1.495(+) | 1.665(+) | / |
| M2 | 0.267(−) | 0.282(−) | / | |
| M3 | 1.565(+) | 1.623(+) | / | |
| M4 | 0.288(−) | 0.308(+) | / | |
| M5 | 0.271(−) | 0.295(−) | / | |
| M6 | 0.316(−) | 0.365(+) | / | |
| FMDV-FLAG | M7 | 1.622(+) | / | 1.517(+) |
| M8 | 0.248(+) | / | 0.272(−) | |
| M9 | 0.127(−) | / | 0.134(−) | |
| M10 | 0.309(−) | / | 0.320(−) | |
| M11 | 1.679(+) | / | 1.868(+) | |
| M12 | 0.255(−) | / | 0.295(−) | |
| r-HN | M13 | 1.695(+) | 0.265(−) | 0.237(−) |
| M14 | 0.267(−) | 0.282(−) | 0.203(−) | |
| M15 | 1.565(+) | 0.223(−) | 0.166(−) | |
| M16 | 0.288(−) | 0.308(−) | 0.240(−) | |
| M17 | 0.271(−) | 0.295(−) | 0.272(−) | |
| M18 | 0.316(−) | 0.365(−) | 0.248(−) | |
| Mean Neg.Control(n = 4) | 0.308 | 0.272 | 0.255 | |
| Mean Pos. Control | 1.445 | / | / | |
| Cutoff value | 0.525 | 0.571 | 0.536 |
The cutoff value of tag antibodies = 2.1×mean negative control. The cutoff value of 3ABC antibodies was calculated as previously described [37].
+, represents antibody positive; −, represent antibody negative.