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. 2012 Jul 27;7(7):e41569. doi: 10.1371/journal.pone.0041569

Figure 4. The deduced protein sequence of Eavas and schematic of the Eavas domain structure.

Figure 4

A. The deduced Eavas amino acid sequence. *, motifs conserved among DEAD-box protein family members; black dotted line, CCHC zinc fingers; black box with dotted line, RGG motif; black box, location of the antigen sequence used for antibody production. B. Schematic figure depicting the Eavas domain structure and regions that are highly homologous among the Vasa subfamilies. Vertical lines mark the positions of the nine conserved DEAD-box motifs. C. A phylogenetic tree comparing the amino acid sequences of Vasa- and PL10-related proteins from various taxa. The region used for this analysis corresponds to the sequence AFLLPV…LDEA (from 276 to 385 residues of Eavas), where are available and comparable region among various taxa. The sequences were aligned by a multiple sequence alignment using MUSCLE. The phylogenetic tree was constructed using the neighbor-joining method. The number at each node represents the bootstrap probability (%); the branches shown correspond to values of 50% and higher. The names and corresponding GenBank accession numbers of the proteins analyzed are as follows: Acropora CnVas (Acropora digitifera, BAB13683), Euphyllia Eavas (Euphyllia ancora, JQ968407), Nematostella Nvvas1 (Nematostella vectensis, AAW29073), Nematostella Nvvas2 (Nematostella vectensis, AAW29074), Tima Cnvas1 (Tima Formosa, BAB13687), Hydractinia Cnvas (Hydractinia echinata, BAB13686), Hydra Cnvas1 (Hydra vulgaris, BAB13307), Hydra Cnvas2 (Hydra vulgaris, BAB13308), Ephydatia PoVAS1 (Ephydatia fluviatilis, BAB13310), Ciona Ci DEAD1 (Ciona intestinalis, BAA36710), Xenopus XVLG1 (Xenopus laevis, NP_001081728), Danio vasa (Danio rerio, AAI29276), Gallus Cvh (Gallus gallus, BAB12337), Rattus VLG (Rattus sp., AAB33364), Mus Mvh (Mus musculus, BAA03584), Schistocerca vasa-like (Schistocerca gregaria, AF510054), Drosophila vasa (Drosophila melanogaster, NP_723899), Bombyx BmVLG (Bombyx mori, BAA19572), Acropora CnPL10 (Acropora digitifera, BAB13676), Euphyllia EaPL10 (Euphyllia ancora, JQ968406), Nematostella NvPL10 (Nematostella vectensis, AAW29072), Hydractinia CnPL10 (Hydractinia echinata, BAB13679), Hydra CnPL10 (Hydra vulgaris, BAB13306), Ephydatia PoPL10 (Ephydatia fluviatilis, BAB13309), Danio pl10 (Danio rerio, NP_571016), Xenopus ddx3x (Xenopus laevis, NP_001080283), Mus PL10 (Mus musculus, AAA39942), Mus p68 (Mus musculus, CAA46581), and Saccharomyces p68 (Saccharomyces cerevisiae, CAA36874).