Skip to main content
. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Jun 27;263(1):122–130. doi: 10.1016/j.taap.2012.06.013

Figure 1.

Figure 1

Effect of GSPs and 5-aza-dc on global DNA methylation levels in human squamous cell carcinoma A431 and SCC13 cells. (A) Comparative levels of global DNA methylation in NHEK, A431 and SCC13 cells. Data are presented in terms of percent of NHEK, which were used as normal control cells. Significantly higher level of global DNA methylation level versus NHEK, *P<0.001. (B & C) Effect of GSPs on the global DNA methylation levels in A431 and SCC13 cells. A431 and SCC13 cells were treated with various concentrations of GSPs (0, 5, 10, 15 and 20 μg/ml) for 3 and 5 days and the levels of global DNA methylation were determined using Global DNA Methylation kit. 5-aza-dc is a well known inhibitor of DNA methylation and was used as a positive control. Data are presented in terms of percent of control (non-GSPs-treated) group, which was assigned a value of 100%, and as means ± SD, n=3. Significant difference versus non-GSPs-treated controls, *P<0.001, **P<0.01.