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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Jun 27;263(1):122–130. doi: 10.1016/j.taap.2012.06.013

Figure 3.

Figure 3

Treatment of A431 or SCC13 cells with GSPs or 5-aza-dc decreases DNMT activity and reduces the levels of mRNA and protein expressions of DNMT1, DNMT3a and DNMT3b. (A) Total DNMT activity in nuclear extracts was determined using the DNA Methyltransferase Activity Assay Kit. Data are presented in terms of percentage versus non-GSPs-treated controls, which was assigned a value of 100% and as means ± SD from three independent experiments. (B) Quantitative real-time PCR analyses of mRNA expressions of DNMT1, DNMT3a and DNMT3b in A431 and SCC13 cells after treatment of cells with GSPs or 5-aza-dc for 5 days. The results are presented as the expression of the individual mRNA with normalization to β-actin, and as mean values ± SD from three independent experiments. Significant difference versus non-GSPs-treated controls, *P<0.001. (C) The levels of DNMT1, DNMT3a and DNMT3b in cell lysates of A431 and SCC13 cells were determined using western blot analysis after treating the cells with GSPs or 5-aza-dc for 5 days. Representative blots are shown from three independent experiments.