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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Jun 27;263(1):122–130. doi: 10.1016/j.taap.2012.06.013

Figure 5.

Figure 5

Effect of GSPs or TSA on acetylation of histones in human skin cancer cells. (A) The basal levels of HDAC3 and HDAC4 were determined in A431 and SCC13 cells using western blot analysis. (B) The effect of GSPs and 5-aza-dc was determined on the levels of methyl H3 (Lys 9) in SCC13 cells. Cells were treated for 5 days with GSPs or 5-aza-dc, and cell lysates were prepared for western blot analysis. Cells treated with 5-aza-dc served as a positive control. (C) Cells were treated with GSPs or TSA for 5 days, and cell lysates were analyzed for modifications in histone acetylation levels using western blotting. Representative blots are shown from 3 independent experiments. (D) Effect of GSPs on HAT activity in SCC13 cells after treating the cells for 5 days, as detailed in Materials and methods. Significant increase versus non-GSPs-treated control, **P<0.05, *P<0.001.