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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Syst Appl Microbiol. 2012 Apr 21;35(8):496–502. doi: 10.1016/j.syapm.2012.03.004

Figure 1. The power of combinations.

Figure 1

With a relatively small number of fluorophores, it is possible to create many different specific combinations. When the number of dyes, n, is 6, and the combination size, k, is set to 2 there exist 15 different combinations. The binary label restriction gives a more robust assay because the a priori knowledge that all cells in an image are labeled with exactly two fluorophores is used when assigning objects their label-type.