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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Syst Appl Microbiol. 2012 Apr 21;35(8):496–502. doi: 10.1016/j.syapm.2012.03.004

Figure 2. Spectral Imaging and Linear Unmixing of microbes.

Figure 2

(A). 24-channel spectral image of a mixture of E. coli cells labeled either singly or in binary combination with BODIPY-FL and Oregon Green-514. (B). Emission spectra of the two fluorophores as recorded on the instrument used to acquire the image in (A). (C). Pseudocolor image after application of linear unmixing. BODIPY-FL intensity is colored green and Oregon Green 514 is red. Cells labeled with a combination of both fluorophores appear yellow in this merged image.