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. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: FEBS Lett. 2012 Jun 20;586(16):2294–2299. doi: 10.1016/j.febslet.2012.06.016

Figure 6. Effects of paxillin LIM-3,4 domain expression on OxPAPC-induced endothelial barrier enhancement and Rac pathway activation in pulmonary endothelial cells.

Figure 6

HPAEC were transfected with full length GST-tagged paxillin or its LIM-3,4 domain. After 48 hrs of transfection, the cells were stimulated with vehicle or OxPAPC (15 mg/ml). A: Changes in transendothelial electrical resistance were monitored over time. B: Site-specific phosphorylation of PAK and cortactin was detected by Western blot. Equal protein loading was confirmed by determination of β-tubulin and paxillin content in total cell lysates. Expression level of GFP-tagged full length paxillin was detected in whole cell lysates using paxillin monoclonal antibody antibody (clone 349), GST- LIM-3,4 was detected using GST antibody.