Skip to main content
. Author manuscript; available in PMC: 2013 Jul 30.
Published in final edited form as: FEBS Lett. 2012 Jun 7;586(16):2232–2238. doi: 10.1016/j.febslet.2012.05.039

Figure 1. Effects of ProRS and TyrRS on aminoacylation by LeuRS.

Figure 1

T. kodakarensis total tRNA (2 mg/ml) was aminoacylated under standard conditions (7 μl samples) in the presence of LeuRS alone (80 nM) (◻) or with addition of A, ProRS (800 nM) (∎) or B, TyrRS (3.2 μM) (◆). Non-specific increase in the activity of aaRSs were excluded by adding BSA in the reaction mixtures. Further addition of BSA did not change the activities of the aaRSs being monitored (data not shown).