Table 1. Mutation rates and sequencing data for the yeast strains used in this study.
URA3 orientation | OR1 | OR2 | OR1 | OR2 |
---|---|---|---|---|
Strain | POL2+ rnh201Δ | pol2–4 rnh201Δ | ||
Mutation rate (× 10−8) | 3.1 | 3.7 | 21 | 36 |
95% CI | (2.7–3.5) | (3.3–4.5) | (18–26) | (29–45) |
ura3 mutants sequenced | 251 | 273 | 216 | 200 |
Single base substitutions | 86 | 89 | 154 | 57 |
Single base indels | 27 | 14 | 26 | 15 |
Total 2–5 base deletions | 25 | 96 | 11 | 27 |
Other* | 19 | 13 | 3 | 0 |
Other mutations include mutations involving multiple bases (deletions of >5 bases, insertions of >1 base, duplications and complex mutations). Sequencing data for the POL2+ rnh201Δ strain is from [11].