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. Author manuscript; available in PMC: 2012 Jul 28.
Published in final edited form as: Expert Rev Proteomics. 2011 Apr;8(2):157–163. doi: 10.1586/epr.11.14

Figure 1. Typical workflow for a large-scale phosphoproteomics experiment.

Figure 1

This workflow combines a number of different approaches that have been used to successfully identify and quantify phosphopeptides from the kidney collecting duct.

HCl: Hydrochloride; IMAC: Immobilized metal affinity chromatography; iTRAQ: Isobaric tags for relative and absolute quantitation; LC-MS/MS: Liquid chromatography-tandem mass spectrometry; MOAC: Metal oxide affinity chromatography; MS: Mass spectrometry; SILAC: Stable isotope labeling by amino acids in cell culture.