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Immunofluorescence analysis of NIS expression in FRTL-5 cells. FRTL-5 cells were maintained in a medium without TSH for 7 days. The medium was then replaced with the same medium (A) or a medium containing 5 mM PTU (B), 5 mM MMI (C), or 1 mU/mL TSH (D), for 48 hours. Cells were incubated first with anti-NIS Ab for 1 hour, and then with fluorescent-labeled goat anti-rabbit Ab. Bar: 50 μm.
