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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: J Neurosci Methods. 2011 Jun 25;200(2):95–105. doi: 10.1016/j.jneumeth.2011.06.006

Figure 3. Immunofluorescence staining of mGFP-expressing PNs in dissociated cerebellar culture using anti-Calbindin-D-28K antibody.

Figure 3

Dissociated cerebellar cells were nucleofected with 10 μg of pL7-mGFP, cultured for 13 DIV, and subjected to immunofluorescence staining using an antibody against the PN-specific protein Calbindin-D-28K. Images show maximum projections of stacks of Z-planes acquired using confocal microscopy and correspond to the anti-Calbindin immunofluorescence signal (A), the mGFP signal (B), and the superimposition of these images (C). Size bar, 50 μm.