Table 1.
Anticonvulsant effect of the subcutaneous administration of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion of Spongia officinalis in the PTZ model in mice
| Treatment | Dose (mg/kg) | Onset of first clonus (s) | Duration (s) | Seizure protection (%) | Mortality protection (%) |
|---|---|---|---|---|---|
| Control (saline 10 ml/kg) |
- |
180.16 ± 53.66 |
15 ± 1.16 |
0 |
0 |
| Crude extract |
100 |
149 ± 35.77ns |
12 ± 87.24ns |
0 |
0 |
| 200 |
251 ± 17.88 ns |
10.7 ± 3.57ns |
0 |
0 |
|
| 400 |
600 ± 0** |
5 ± 2.68** |
30 |
0 |
|
| F1 |
200 |
251 ± 9.83* |
18 ± 3.57ns |
0 |
o |
| F2 |
50 |
247 ± 17.88 ns |
8.7 ± 1.97** |
0 |
0 |
| 100 |
800 ± 17.88** |
4 ± 0.71** |
20 |
0 |
|
| 200 |
1200 ± 89.44** |
1 ± 0.89** |
50 |
30 |
|
| F3 |
200 |
249 ± 10.73 ns |
13 ± 1.07ns |
0 |
0 |
| Phenobarbital (reference drug) | 120 | - | - | 100 | 100 |
Values are expressed as mean ± s.e.m. *P < 0.01, **P < 0.001, ns: not significant. n = 6 animals.