Table 3.
Analgesic effect of the subcutaneous administration of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion of Spongia officinalis in the PBQ writhing test in mice
| Treatment | Dose (mg/kg) | Number of writhes ± s.e.m. |
inhibition of writhing (%) |
|---|---|---|---|
| Control (saline 10 ml/kg) |
- |
39 ± 2.6 |
- |
| Crude extract |
100 |
14.33 ± 5.16** |
63.24 |
| 200 |
12.33 ± 4.8** |
68.37 |
|
| 400 |
9.66 ± 5.08** |
75.21 |
|
| F1 |
200 |
12 ± 3.74** |
69.23 |
| F2 |
50 |
11 ± 4.19** |
71.79 |
| 100 |
8 ± 3.4** |
79.48 |
|
| 200 |
7 ± 2.36** |
82.05 |
|
| F3 |
200 |
11.33 ± 4.32** |
70.94 |
| Acetylsalicylate of lysine (reference drug) | 200 | 10 ± 2.6** | 74.35 |
Values are expressed as mean ± s.e.m. **P < 0.001. n = 6 animals.