Skip to main content
. Author manuscript; available in PMC: 2013 Jan 2.
Published in final edited form as: ChemMedChem. 2011 Dec 14;7(1):79–84. doi: 10.1002/cmdc.201100457

Figure 1. Flow cytometry results of aptamer binding with MCF-10AT1 cells under different conditions.

Figure 1

All the aptamers were labeled with biotin. A: KMF2-1a shows stable binding to target cells at 4°C. Red line: MCF-10AT1 cells. Green line: library aptamers as negative control. Blue line: 30mins after KMF2-1a was incubated with target cells at 4°C. Purple line: 2h after KMF2-1a was incubated with target cells at 4°C. B: KMF2-1a can still bind to target cells at 37°C. Red line: MCF-10AT1 cells. Green line: library aptamers as negative control. Blue line: 30mins after KMF2-1a was incubated with target cells at 37°C. C: Negative control MCF-10A1 cells could not bind with KMF2-1a. Red line: MCF-10A1 cells. Green line: library aptamers. Blue line: MCF-10A1 cells incubated with KMF2-1a at 4°C for 30mins. D: Trypsin removed specific binding of KMF2-1a. Red line: MCF-10AT1 cells. Green line: library (negative control) incubated with target cells without trypsin treatment at 4°C. Blue line: KMF2-1a incubated with target cells without trypsin treatment at 4°C. Purple line: KMF2-1a-target cell incubation after trypsin treatment at 4°C.