Table 1.
NO2- production from furoxans after 24 h with excess cysteine
| Compound | NO2- production (μM)a | NO2- production as % (mol/mol)b |
|---|---|---|
| 2a | 9.2 (±0.1) | 18.4 % |
| 2b | 5.1 (±0.1) | 10.4 % |
| 2c | 14.8 (±0.8) | 29.6 % |
| 3 | 40.1 (±0.8) | 80.2 % |
| 4 | 6.3 (±0.1) | 12.6 % |
| 5 | 9.7 (±0.1) | 19.4 % |
| 6a | 6.8 (±0.1) | 13.6% |
| 6b | 3.9 (±0.7) | 7.8 % |
| 6c | 21.8 (±0.5) | 43.6 % |
| 7a | 1.0 (±0.4) | 2.0 % |
| 7b | 1.8 (±0.1) | 3.5 % |
| 7c | 1.3 (±0.1) | 2.5 % |
| 9a | 1.2 (±0.2) | 2.4 % |
| 9b | 0.5 (±0.1) | 1.0 % |
| 10 | 0.0 (±0.1) | 0 % |
| 11 | 29.1 (±0.3) | 58.2 % |
a
Quantitative amount of NO2- produced from 250 μM of compound after 24 h in the presence of excess cysteine (2.5 mM) in PBS (50 mM, pH 7.4) at 37 °C; quantified using calibration curve generated with NaNO2- using the Griess assay.
b
NO2- production represented as % mol/mol.