Table 2.
Cysteine remaining after 2 h incubation with furoxans under hypoxic conditions
| Compound | Cys as free thiol (mM)a | Cys depletion as % (mol/mol)b |
|---|---|---|
| - | 2267.2 (± 153.7) | - |
| 4 | 1788.8 (± 18.7) | 21.1 % |
| 5 | 1410.1 (± 23.8) | 37.8 % |
| 6a | 1790.8 (± 38.6) | 21.0 % |
| 6b | 1830.4 (± 16.6) | 19.2 % |
| 6c | 1995.2 (± 26.1) | 12.0 % |
| 7a | 1724.4 (± 26.1) | 23.9 % |
| 7b | 1507.2 (± 29.5) | 33.5 % |
| 7c | 1620.4 (± 26.6) | 28.5 % |
| 9a | 1812.1 (± 33.4) | 20.1 % |
| 9b | 1724.4 (± 59.0) | 23.9 % |
| 10 | 1742.8 (± 23.3) | 23.1 % |
a
Free cysteine remaining in incubations containing furoxans (250 μM) and cysteine (2.5 mM) after 2 h under hypoxic conditions at 37 °C in PBS (50 mM, pH 7.4)/ CH3CN (1:1), with 1 mM EDTA.
b
Cysteine depletion relative to the control, represented as percent cysteine depletion mol/mol.