Skip to main content
. Author manuscript; available in PMC: 2013 Jul 3.
Published in final edited form as: Cell Metab. 2012 Jun 21;16(1):104–112. doi: 10.1016/j.cmet.2012.05.010

Figure 3. Leptin Stimulates αAMPK Serine485/491 Phosphorylation through PI3K-AKT Signaling.

Figure 3

(A and B) i.c.v. injection of vehicle (DMSO) or LY294002 (0.1 nmol; 30 min) followed by saline (−) or leptin (20 ng; 3 hr) (+) in overnight-fasted mice (n = 7/group). ARC (A) and VMH/DMH (B) were analyzed for α2AMPK activity and αAMPK serine485/491/α2AMPK.

(C) GT1-7 neurons were treated with DMSO or LY294002 (20 μM) and 0.5 μg/ml leptin (+) or vehicle (−) for 2 hr and analyzed for α2AMPK activity and αAMPK serine485/491/α2AMPK.

(D) GT1-7 neurons were transfected with CA- or DN-AKT or vector (Vec) and analyzed for α2AMPK activity and αAMPK serine485/491/α2AMPK.

(E) GT1-7 neurons were transfected with DN-AKT or vector and treated with 0.5 μg/ml leptin (+) or vehicle (−) for 2 hr. Cell extracts were analyzed for α2AMPK activity and αAMPK serine485/491/α2AMPK. *p < 0.05 versus empty vector control. #p < 0.05 versus empty vector leptin.

(F) pS6K/S6K and pS6/S6 in hypothalamic nuclei after saline or leptin injection (20 ng i.c.v.; 3 hr) in overnight fasted mice (n = 6/group). *p < 0.05 versus saline.

Data in all panels are shown as means ± SEM. See also Figure S3.