Figure 7.

Effect of generated kinins on HUVECs intracellular Ca²⁺mobilization in the presence of the B1 receptor antagonist Des-Arg⁹Leu⁸-BK. Malaria parasites (10⁴ cells) were incubated with 20 μg of HK for 30 minutes, and extracellular medium was then added to HUVECs loaded with the Fluo-3 AM fluorescent calcium probe and previously incubated for 10 min with Des-Arg⁹Leu⁸-BK (4 μM). (A) Intracellular calcium fluorescence of cells incubated with HK (20 μg), 10 μl of parasite culture medium without HK incubation (Pf), 10 μl of parasite culture medium after incubation with 1.9 μg/μL of HK for 30 min (Pf + HK) and thapsigargin (THG 5 μM), a ER Ca²⁺-ATPase inhibitor. Each line represents results for a single cell; (B) Time lapse calcium fluorescence images of HUVECs measured by confocal microscopy, with the times of addition of components indicated.