Table 1.
Amoeboid microglia isolation procedure
| Step | Manipulation | Time |
|---|---|---|
| 1. |
Dissection and mechanical dissociation of cerebral tissue from newborn rat |
1 hour |
| 2. |
Incubate in flask with defined medium, 37 °C |
1 day |
| 3. |
Agitation by rotary shaker, 180 rpm, 37 °C |
15 hours |
| 4. |
Adhesion incubation in flask, 37 °C |
1 to 3 hours |
| 5. |
Agitation by hand, 20 °C |
2 to5 minutes |
| 6. |
Adhesion incubation in flask, 37 °C |
1 to 3 hours |
| 7. | Agitation by hand, 20 °C | 2 to 5 minutes |
The table is adapted from Guilian and Baker, 1986 [8]. The table describes a common procedure for obtaining primary microglial cells.