Table 1.
Primers used for QPCR
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
|
AMPK |
AAGATCGGACACTACGTCCTG |
TGCCACTTTATGGCCTGTCAA |
|
Akt2 |
ACGTGGTGAATACATCAAGACC |
ACCCAATGAAAGATCCATCACTC |
|
CD36 |
ATGGGCTGTGATCGGAACTG |
AGCCAGGACTGCACCAATAAC |
|
CD68 |
GGACCCACAACTGTCACTCAT |
AAGCCCCACTTTAGCTTTACC |
|
FAS |
GGAGGTGGTGATAGCCGGTAT |
TGGGTAATCCATAGAGCCCAG |
|
HMGCR |
TGTTCACCGGCAACAACAAGA |
CCGCGTTATCGTCAGGATGA |
|
IRS2 |
TCTACACCCGAGACGAACACT |
TGGGCCTTTGCCCGATTATG |
|
LDLR |
TGACTCAGACGAACAAGGCTG |
ATCTAGGCAATCTCGGTCTCC |
|
LPL |
TTGCCCTAAGGACCCCTGAA |
ACAGAGTCTGCTAATCCAGGAAT |
|
LXRα |
CTCAATGCCTGATGTTTCTCCT |
TCCAACCCTATCCCTAAAGCAA |
|
MAC1 |
ATGGACGCTGATGGCAATACC |
TCCCCATTCACGTCTCCCA |
|
MMP3 |
ACATGGAGACTTTGTCCCTTTTG |
TTGGCTGAGTGGTAGAGTCCC |
|
PPARγ |
GGAAGACCACTCGCATTCCTT |
GTAATCAGCAACCATTGGGTCA |
|
SCD-1 |
TTCTTGCGATACACTCTGGTGC |
CGGGATTGAATGTTCTTGTCGT |
|
SREBP1c |
GATGTGCGAACTGGACA |
CATAGGGGGCGTCAAACAG |
| SREBP2 | TGGGCGATGAGCTGACTCT | ACTGTAGCATCTCGTCGATGT |
AMPK: 5' adenosine monophosphate-activated protein kinase, Akt2: protein kinase B beta, CD36: CD36 antigen, CD68: CD68 antigen, FAS: fatty acid synthase, HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase, IRS2: insulin receptor substrate 2, LDLR: low-density lipoprotein receptor, LPL: lipoprotein lipase, LXRα: liver X receptor alpha, MAC1: macrophage-1 antigen, MMP3: matrix metalloprotease 3, PPARγ: peroxisome proliferator activated receptor gamma, SCD-1: stearoyl CoA desaturase-1, SREBP: sterol regulatory element binding protein.