Table 2.

Nucleotide substitutions in theenvpartial sequence among new Italian USUV strains and reference sequences

USUV strains	Genbank accession number	Nucleotide positiona
		1209	1242	1251	1294	1335	1344	1351	1458	1470	1554	1731	1771	1785	2073	2076	2121	2181	2196	2463	2466
Vienna2001	AY453411	g	g	c	c	c	g	a	a	g	a	t	t	t	c	c	c	c	c	a	c
Budapest	EF206350	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	t	.	.	.
USU629_05	EF393681	.	.	.	.	.	.	.	g	a	.	.	.	.	.	.	.	.	.	.	.
USU502_03	EF078297	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
USU281_03	EF078294	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
USU623_04	EF078300	.	.	.	.	t	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
USU618_04	EF078299	.	.	.	.	.	.	.	.	.	g	.	.	.	.	.	.	.	.	.	.
USU499_03	EF078296	.	.	.	.	.	.	.	.	.	.	.	.	.	t	.	.	.	.	.	.
USU450_03	EF078295	.	a	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
USU604_05	EF393680	a	.	t	.	.	.	g	.	.	.	c	.	.	.	.	.	.	.	.	.
USU588_05	EF393679	a	.	t	.	.	.	g	.	.	.	c	.	.	.	.	.	.	.	.	.
USU589_05	EF078301	a	.	t	.	.	.	g	.	.	.	c	.	.	.	.	.	.	.	.	.
USU338_04	EF078298	.	.	.	.	.	.	.	.	.	.	.	c	c	.	.	t	.	.	.	t
USU090_10	JN257974	.	.	.	t	.	a	.	.	a	.	.	.	.	.	.	.	.	.	g	.
USU181_09	JN257976	.	.	.	.	.	.	.	.	.	.	.	.	.	.	t	.	.	.	g	.
USU173_09	JN257975	.	.	.	.	.	.	.	.	.	g	.	.	.	.	.	.	.	t	.	.

Dots indicate identity respect to Vienna2001 reference strain. The unique non-synonymous substitution at position 1351 is reported in bold.

^a Nucleotide positions refer to the complete genome sequence of USUV Vienna 2001 (AY453411).