Figure 6.

Effects of silencing of PLCG2 and CALM1 expression on viability of cancer cells under higher dose of paclitaxel. 50,000 of HeLa cells from the exponentially growth phase were seeded in each of the wells of a 24-well plates the day before the siRNA/apatite complexes were prepared by mixing 3 μl of 1 M CaCl₂ with 10 nM of siRNA in 1 ml of fresh serum-free HCO₃^- (44 mM)-buffered DMEM medium (pH 7.5) and incubating at 37 °C for 30 min. The medium containing the siRNA/apatite complexes supplemented with 10% FBS had been added onto the rinsed cells either with or without 1 μM of paclitaxel before the cells were cultured consecutively for 48 h and the assessment on cell viability was carried out. Each experiment was done in triplicate and the data represent mean value ± SE (n = 3).