Figure 3.

Hydrogen peroxide inhibits vesicle transport.A. Representative kymographs illustrating the transport of Golgi-derived vesicles before and at various time points after exposure to 100 μM hydrogen peroxide. B. Hand-traced lines from the above kymographs. C&D. Quantitative data of Golgi-derived vesicle transport following hydrogen peroxide treatment (n = 20 cells). For each cell, the velocity and event number were normalized to pretreatment levels. C. Velocities of Golgi-derived vesicles in both directions decreased over time. D. The anterograde event numbers of Golgi-derived vesicles was inhibited to around 40% by the end of one hour while the number of retrograde events did not change. Under this imaging condition, both velocities and event numbers of Golgi-derived vesicles transport in control neurons remain stable (control, open circle; hydrogen peroxide treatment, filled circle). The transport at each time point was compared with pre-treatment levels using paired student t-test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar indicates SD.