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Wnt ligands in the diastema. Dark field (A, B, C, D, and E) and light field (A’, B’, C’, D’, and E’) images of radioactive in situ hybridization on sagittal sections in wild-type mice at E14.5. Weak expression of Wnt4 and Wnt10b (A, A’, D, and D’) was detected in diastema epithelium. Wnt5a expression (B and B’) was observed in diastema mesenchyme. Wnt6 and Wnt11 (C, C’, E, and E’) were expressed in both diastema epithelium and mesenchyme. Arrows indicate rudimentary tooth bud.
