Figure 5.

Dkk expression in the diastema. Dark field (A, B, C, D, and E) and light field (A’, B’, C’, D’, and E’) images of radioactive in situ hybridization on sagittal sections in wild-type mice at E14.5. The expression of Dkk1 (A and A’) was found in presumptive maxillary bone adjacent to the diastema in wild-type. Normal expression of Dkk1 (B and B’) was detected in Lrp4^Hypo/Hypo mutant. Dkk2 (C and C’) and Dkk3 (D and D’) were expressed in upper diastema mesenchyme. Dkk4 expression (E and E’) could not be detected in the diastema. Arrows indicate rudimentary tooth bud.