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. 2012 Jul 23;10(7):1605–1618. doi: 10.3390/md10071605

Figure 3.

Figure 3

Overlaid 2D 1H-15N HSQC spectrum of uniformly 15N-labelled wild-type rAPETx2 (blue) and a F15A mutant (red). Residues whose chemical shifts are most affected by the mutation of Phe15 to Ala are highlighted with green circles while the peak corresponding to F15 in the wild-type toxin is shown in a red box. The horizontal line connects the two peaks from the sidechain amide group of Asn8, and “W14 sc” indicates the peak from the sidechain indole NH group of Trp14.