Figure 3.
C57BL/6 Mice were fed alcohol (EtOH) for 6 weeks and were exposed to 12.5% HDE intranasally for 3 weeks (n = 5). BAL fluid was collected from these mice and cytokine levels were quantified via ELISA. (A) IL-6 was ~50% less in the alcohol + HDE group as opposed to the HDE only group (46.8/96.1 pg/mL);(B) KC quantification detailed a statistically significant reduction in the alcohol + HDE group compared to HDE only group (34.1/79.6 pg/mL); (C) MIP-2 quantification detailed a statistically significant reduction in the alcohol + HDE vs. the HDE only group (23.1/31.5 pg/mL) (* p < 0.05 vs. control ANOVA, Bonferroni post-test; #p < 0.05 vs. HDE, ANOVA, Bonferroni post-test).