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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Biochem J. 2011 Jul 15;437(2):289–299. doi: 10.1042/BJ20110587

Fig. 5. Reduced expression of PKCζ by antisense oligos attenuates tight junction integrity in Caco-2 cells.

Fig. 5

A & B: Caco-2 cells were transfected with missense oligo (MS) or two different antisense oligos (AS-1, AS-2), and the levels of PKCζ and PKCλ were measured by immunoblot analysis. Band densities evaluated by densitometric analysis (B). Values are mean ± sem (n = 6). Asterisks indicate the values that are significantly (P<0.05) different from corresponding values for MS-transfected cells. C & D: Barrier function (C) and inulin permeability (D) were measured on day 3 after seeding. Values are mean ± sem (n = 6). Asterisks indicate the values that are significantly (P<0.05) different from corresponding values for MS-transfected cells. E: Caco-2 cell monolayers transfected with MS or AS-2 were fixed and stained for occludin and ZO-1 by immunofluorescence method.