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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Biochem J. 2011 Jul 15;437(2):289–299. doi: 10.1042/BJ20110587

Fig. 9. PKCζ-PS attenuates tight junction integrity in mouse ileum.

Fig. 9

Mouse ileal strips were incubated with or without varying doses of PKCζ-PS for one hour. A, B: Cryosections of tissues were stained for occludin and ZO-1 (A) or E-cadherin and β-catenin (B) by immunofluorescence method. C, D: Triton-insoluble (C) and Triton-soluble (D) fractions prepared from mucosal scrapings of ileum were immunoblotted for different proteins. E: At the end of incubations with PKCζ-PS medium was analyzed for LDH activity. For positive control tissues were incubated with 0.1% Triton X100. LDH values for tissues incubated with no inhibitor or Triton X100 were subtracted from experimental values. Values are mean ± sem (n = 4).