Figure 3. Effect of NAC on safingol-based drug combinations observed in various cancer cell lines. (A-D) Molar ratios (4:1) of safingol/carboplatin, safingol/doxubicin, safingol/gemcitabine and safingol/vincristine were tested in the absence and presence of 10 mM NAC, with the exception of for U937 cells where NAC was 5 mM. The cells were treated for 48 h. The results were obtained from cells treated with 5 µM safingol, with the exception of for KB cells where 10 µM safingol was used. Data shown are the means ± SEM from three independent experiments. ^*NAC-treated group compared to non-NAC group.