Figure 2. Co-localization of B⁰AT1 and APN.

HEK-293 cells in eight-well microscope slide dishes were transfected with plasmid DNA (constructs used are indicated on the left-hand side) when cells had achieved >90% confluency. The eGFP fluorescence was visualized with a Leica SP5 confocal system and processed with LAS AF software. Indicated above the panels is whether or not cells were co-transfected with the pcDNA mammalian expression vector encoding the trafficking protein collectrin. The white scale bars indicate 25 μm. All images were taken at ×63 magnification.