Table 2. Kinetic properties of B0AT1/APN co-expressing oocytes.
X. laevis oocytes where injected with the transporter and auxiliary protein indicated. The Na+-induced currents were recorded as outlined in Figure 4. Each fitted data point represents the mean±S.D. (n=6–8, e=3).
Transporter | Substrate | Auxiliary protein | Imax (nA±S.D.) | Apparent Km (mM±S.D.) |
---|---|---|---|---|
B0AT1 | Leucine | Collectrin | 523±57 | 1.35±0.17 |
APN | 17.4±1.5 | 0.63±0.07 | ||
B0AT1 | Glutamine | Collectrin | 720±111 | 2.56±0.54 |
APN | 27±5.6 | 1.17±0.18 | ||
B0AT1 | Phenylalanine | Collectrin | 577±69 | 8.66±1.83 |
APN | 22±4 | 4.00±0.43 |