Table 3. Validation of experimental apparent K_m values for B⁰AT1 and APN co-expression.

The kinetic parameters apparent K_m and I_max were calculated using electrophysiological recordings of the proteins indicated expressed in X. laevis oocytes (Figure 4A). V_max (mmol/min per oocyte) values were calculated from I_max values, and V_predicted was calculated by substituting these values into the Michaelis–Menten function at 10 mM or 100 μM substrate concentrations ([S]). V_experimental represents the mean±S.E.M. from three experiments using electrophysiological and uptake experiments (Figure 3D). All rate (V) values were calculated as mmol/min per oocyte and fold-change was calculated relative to B⁰AT1-expressing oocytes.

			[S]=10 mM		[S]=100 μM
Protein(s) expressed in oocytes	Apparent Km (mM±S.D.)	Imax (nA±S.D.)	Vpredicted fold-change	Vexperimental fold-change	Vpredicted fold-change	Vexperimental fold-change
B0AT1	1.02±0.06	8.09±2.33	1	1	1	1
B0AT1/APN	0.52±0.02	18.78±1.86	2.42	2.16±0.18	4.18	4.70±0.39
B0AT1/ACE2	1.09±0.04	458.90±85.28	56.53	49.28±5.66	53.40	45.60±9.56