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. 2012 Jul 27;446(Pt 1):23–36. doi: 10.1042/BJ20112024

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Haemotoxylin-and-eosin-stained micrographs of C2C12 differentiated myotubes treated for 24 h with low-serum medium (Ctrl), CM, CM pre-incubated with anti-Mstn antibody (CM+anti-Mstn) and CM pre-incubated with anti-activin A antibody (CM+anti-activin A). Scale bars represent 100 μm. (B) The line graph represents the myotube area (μm²) of control, CM-, CM+anti-Mstn- and CM+anti-activin A-treated C2C12 myotubes as calculated by Image Pro Plus. (C) Average myotube area (μm²) of C2C12 myotubes treated with control, CM, CM+anti-Mstn and CM+anti-activin A. (D) Immunoblot analysis of Myh and Myl expression in C2C12 myotubes exposed to control, CM, CM+anti-Mstn and CM+anti-activin A. Statistical significance was assessed by Student's t test compared with the control; *P<0.01. Ctrl, control; IB, immunoblot.