Figure 4. Tumor angiogenesis was assessed by immunohistochemical staining with anti-CD31 antibody on paraffin-embedded tumor sections. MVD counting was performed at a magnification of x400. Significantly reduced numbers of blood vessels in tumors treated with (E) LH plus DDP, in comparison with (A) NS, (B) EL, (C) LH, (D) DDP. (F) The effect of combined therapy on MVD is shown. Vessel density was determined by counting per high-power (F) column, the mean of the microvessels per high-power field; bars, means ± SD. LH, liposomal honokiol; DDP, cisplatin; NS, normal saline; EL, empty liposomes and MVD, microvessel density.