Effects of DIM-C-pPhBr on expression of several genes with or without NR4A2 knockdown. Panc28 and Panc1 cells were transfected with siRNAs targeting NR4A2 transcripts (iNurr1) or non-specific control oligonucleotides (iCTL). At 24 hr after transfection, cells were treated with 12 μM DIM-C-pPhBr for 18 and 24 hr. Relative expression levels of NR4A2 (A) and NR4A2-dependent genes vasoactive intestinal peptide (B), osteopontin (C), neuropilin-1 (D) and -2 (E) were determined by real-time PCR analysis as described in the Materials and Methods. Results are expressed as means ± SD for at least three separate determinations for each treatment. *, P < 0.05, treatment vs. solvent control (DMSO). **, P < 0.05, iNurr1 vs. iCTL. #, P < 0.05, knockdown and treatment combination vs. treatment only.