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. 2012 Jun 28;134(29):12016–12027. doi: 10.1021/ja3009693

Figure 3.

Figure 3

Characterization of HSNO/SNO– generated by transnitrosation of S-nitrosoglutathione (GSNO) and H2S. (A) ESI-TOF-MS spectrum of HSNO generated in transnitrosation reaction between GSNO and H2S. Experimental (black) and theoretical (red) isotope distribution of the detected m/z 64 peak of [HSNO + H]+. (B) Real-time FTIR confirms formation of a new nitrosothiol product. Differential IR spectrum of the reaction of 120 mM GSNO and 100 mM Na2S in 300 mM potassium phosphate buffer pH 7.4 (black, after 1 min; red, after 10 min). Inset: Spectral difference between 14N and 15N labeled HSNO/SNO–. (C) 15N NMR spectrum of HSNO/SNO– at pH 7.4. Black: Mixture of 15N-enriched GSNO with nitrite. Red: After addition of equimolar concentration (25 mM) of sulfide. Blue: After 1 h, only the nitrite signal remains. The reaction was performed in 300 mM potassium phosphate buffer, pH 7.4.