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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Neurochem. 2012 Jun 27;122(4):661–671. doi: 10.1111/j.1471-4159.2012.07816.x

Figure 6. Changes in the topology of the plasma membrane at sites of exocytosis.

Figure 6

(A) P/S and P + 2S images were calculated and aligned to the NPY-Cer image at the times indicated. An NPY-Cer–labeled granule undergoes exocytosis between time 0 and 0.45 s. An increase in pixel intensity in the approximate location of the NPY-Cer image stack is observed, which is consistent with a change in orientation of membrane-intercalated diI. In the P + 2S image, a dimming is observed. Circles are centered over the last observed location of the granule. Bar, 1 μm. (B) Membrane P/S and P+2S were followed for almost 60 s. The dotted line at time 0 indicates the frame before fusion (i.e., the last frame in which the NPY-Cer granule was clearly visible). (C) One possible interpretation of the results in A and B is considered. DiI transition dipole moment orientation is indicated by direction of arrows.