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. 2012 Jul 30;2:545. doi: 10.1038/srep00545

Figure 6. Fewer inflammatory monocytes are recruited to the brain of acutely infected SJL mice as compared to B6 mice.

Figure 6

B6 (A, C, E) and SJL (B, D, F) 18 hpi BILs were collected using a 1.080 g/mL Percoll gradient that removed the population of neutrophils while retaining inflammatory monocytes and analyzed by flow cytometry. FSC vs SSC plots revealed a large difference between B6 (A) and SJL mice (B). Gating on CD45hi cells showed that a large population of CD11b+Gr1+1A8− inflammatory monocytes was present in the B6 BILs at 18 hpi (C, E) and this population was substantially attenuated in SJL mice (D,F). While total BILs isolated from the two strains at either 18 or 24 hpi did not differ (G), the total number of inflammatory monocytes was greatly reduced in SJL mice at both timepoints (H). This difference was highly significant, at P<0.001 for both timepoints. The dot plots and quantitation are representative of at least 10 mice per condition in numerous experiments (>5). Graphs represent mean and 95% CI.