FIGURE 2.
Concentrations response profiles for α3β4α5 nAChR expressed as loose subunits. Oocytes injected with RNA for α3, β4, and α5 subunits in a 1:1 or 1:1:1 ratio were perfused with nAChR agonists acetylcholine (10−5.5 to 10−2; n = 6) (A), cytisine (10−6.25 to 10−2.5; n = 6) (B), nicotine (10−6.25 to 10−2; n = 6) (C), or the α3β4 nAChR antagonist mecamylamine (10−8 to 10−4; n = 6) (D). Data points represent averages (±S.E.). Differences in drug potency and efficacy between groups were analyzed using one-way ANOVA with Tukey's post hoc comparison (see “Experimental Procedures”).