FIGURE 4.
α3β4α5 concatemers are expressed as functional pentamers and are not byproducts of fractional assembly. A, co-injection (1:1:1) of loose α3 and β4 nAChR subunits with the α5 gain of function subtype (V9′S) enhances receptor function (p < 0.01; n = 4 oocytes per group). B, overexpression of the α5(V9′S) subunit has no effect on (α3β4)2(β4) concatemer function (n.s.; n = 4 oocytes per group). Panels A and B represent normalized currents (% of α3β4 (1:1) or β4(p5), respectively. Normalized currents were analyzed using one-way ANOVA (see “Experimental Procedures”).