TABLE 3.

Pharmacological parameters calculated from α3β4-only nAChR expressed from different injected subunit ratios

Oocytes injected with RNA for α3 and β4 subunits in a 1:1, 1:20, or 20:1 molar ratio were perfused with the nAChR agonists acetylcholine (10⁻⁶ to 10⁻² m), cytisine (10^−6.25 to 10⁻³ m), nicotine (10^−6.5 to 10^−3.5 m), or the nAChR antagonist mecamylamine (10^−7.5 to 10⁻⁴ m). Data are presented as the mean ± S.E., with numbers of individual oocytes tested (n) as indicated.

Expressed nAChR	Acetylcholine			Cytisine			Nicotine			Mecamylamine
	n	Log EC50	nH ± S.E.	n	Log EC50	nH ± S.E.	n	Log EC50	nH ± S.E.	n	Log IC50	nH ± S.E.
α3β4 (1:1)	6	−4.2 ± 0.02	1.3 ± 0.07	6	−4.7 ± 0.03	1.3 ± 0.13	6	−4.7 ± 0.04	1.3 ± 0.14	6	−5.3 ± 0.04	−1.4 ± 0.18
α3β4 (1:20)	4	−4.3 ± 0.02	0.96 ± 0.07	4	−5.1 ± 0.05	1.1 ± 0.13	4	−4.6 ± 0.05	1.3 ± 0.17	4	−6.0 ± 0.03	−1.2 ± 0.1
α3β4 (20:1)	4	−3.7 ± 0.01	1.8 ± 0.06	4	−4.2 ± 0.03	1.5 ± 0.14	4	−4.3 ± 0.04	2.2 ± 0.3	4	−6.9 ± 0.1	−1.2 ± 0.14