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. 2012 Jun 4;287(30):25151–25162. doi: 10.1074/jbc.M112.379339

TABLE 3.

Pharmacological parameters calculated from α3β4-only nAChR expressed from different injected subunit ratios

Oocytes injected with RNA for α3 and β4 subunits in a 1:1, 1:20, or 20:1 molar ratio were perfused with the nAChR agonists acetylcholine (10−6 to 10−2 m), cytisine (10−6.25 to 10−3 m), nicotine (10−6.5 to 10−3.5 m), or the nAChR antagonist mecamylamine (10−7.5 to 10−4 m). Data are presented as the mean ± S.E., with numbers of individual oocytes tested (n) as indicated.

Expressed nAChR Acetylcholine
Cytisine
Nicotine
Mecamylamine
n Log EC50 nH ± S.E. n Log EC50 nH ± S.E. n Log EC50 nH ± S.E. n Log IC50 nH ± S.E.
α3β4 (1:1) 6 −4.2 ± 0.02 1.3 ± 0.07 6 −4.7 ± 0.03 1.3 ± 0.13 6 −4.7 ± 0.04 1.3 ± 0.14 6 −5.3 ± 0.04 −1.4 ± 0.18
α3β4 (1:20) 4 −4.3 ± 0.02 0.96 ± 0.07 4 −5.1 ± 0.05 1.1 ± 0.13 4 −4.6 ± 0.05 1.3 ± 0.17 4 −6.0 ± 0.03 −1.2 ± 0.1
α3β4 (20:1) 4 −3.7 ± 0.01 1.8 ± 0.06 4 −4.2 ± 0.03 1.5 ± 0.14 4 −4.3 ± 0.04 2.2 ± 0.3 4 −6.9 ± 0.1 −1.2 ± 0.14