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. 2012 Jun 4;287(30):24941–24954. doi: 10.1074/jbc.M112.369355

FIGURE 2.

FIGURE 2.

Increased transcript expression of the Pcdhβ gene cluster is related to absence of GnT-V activity. A, total RNA was isolated from wild-type and GnT-V knock-out tumor cells and used for detection of transcript levels of Pcdhβ genes. B, wild-type her-2 tumor cells were treated with swainsonine (SW; 1 μg/ml) for 24 h, and total RNA was isolated and used for detection of transcript levels of Pcdhβ by qRT-PCR. C, total RNA was isolated from GnT-V KO cells with GnT-V expression and used for detection of transcript levels of Pcdhβ. D, total RNA was isolated from control (scrambled siRNA)- and GnT-V siRNA-transfected MDA-MB231 cells and used for detection of transcript levels of GnT-V and Pcdhβ genes, respectively. For each transcript, the values are normalized to control (Gapdh or Rpl4) and expressed as means with error bars indicating ±1 S.D. of three independent experiments. *, Student's t test, p < 0.05; **, p < 0.01.