FIGURE 5.

MiR-21 is implicated in the regulation of her-2 signaling-mediated gene expression of Pcdhβ cluster. A, total RNA was pooled from four tumors of GnT-V wild-type and knock-out mice, and expression of mature miR-21 was detected by qRT-PCR. B, total RNA was isolated from MDA-MB231 and SK-BR3 tumor cells with and without GnT-V siRNA expression, and expression of GnT-V and mature miR-21 was detected by qRT-PCR. After wild-type her-2 tumor cells were treated with control and anti-miR-21 siRNA oligonucleotides (50 nm) for 48 h, respectively, cells were collected and subjected to detection for transcripts of miR-21 (C), the Pcdhβ cluster (D), and miR-21 target genes as indicated (E). For each transcript, the values are normalized to control (Gapdh or Rpl4) and expressed as means with error bars indicating ±1 S.D. of three independent experiments. *, Student's t test, p < 0.05; **, p < 0.01.