FIGURE 2.

miR-29 suppresses Rybp expression through direct binding to its 3′ UTR. A, a schematic illustration of base pairing between mmu-miR-29c with the 3′ UTR of the mouse Rybp gene. B, a wild type (WT) or mutant Rybp 3′ UTR luciferase reporter was transfected into C2C12 cells along with the indicated precursor miRNA oligos. Luciferase activities were determined at 48 h post-transfection and normalized to Renilla luciferase activity. Data represent the average of three independent experiments ± S.D. C, Rybp mRNA expression was measured in NC, miR-29, anti-NC, or anti-miR-29 transfected C2C12 cells by qRT-PCR and normalized with GAPDH. Expression folds are shown with respect to NC or anti-NC cells where normalized copy numbers were set to 1. D, Rybp protein expression was measured in the above transfected C2C12 cells by Western blotting using α-Tubulin as a loading control. Numbers below indicate densimetric quantification of the Western bands.