FIGURE 2.

Cardiac myocyte-specific cardiolipin synthase transgenic expression regulates myocardial lipidomic flux. A, total cardiolipin content of the cardiac myocyte-specific transgenic CLS mouse in comparison with wild type littermates. B, content of phosphatidylglycerol, the precursor for cardiolipin biosynthesis, was markedly depleted during development and remained at diminished levels in the CLS transgenic mouse. C, in contrast, tetra-18:2 cardiolipin content was dramatically increased starting at postnatal day 14 and maintained in the adult CLS transgenic mouse. Values represent the mean total lipid content or molecular species content (nmol/mg protein) ± S.E. (error bars) (n = 3–4 different hearts/group or isolated mitochondria). D, a 2-fold increase in 18:2-CoA incorporation into cardiolipin, indicative of cardiolipin remodeling, was present after calcium challenge in the CLS transgenic mouse. Black diamonds/bars, wild type mice; white squares/bars, CLS transgenic mice. *, p < 0.05 level; **, p < 0.01 level.